Efek Ekstrak Air Kombinasi Daun Jati Belanda (Guazuma ulmifolia lamk.), Kemuning (Murraya paniculata (l.) Jack), Murbei (Morus alba l.), dan Rimpang Bangle (Zingiber purpureum roxb.) terhadap Derajat Perlemakan Hepar Tikus dengan Diet Tinggi Lemak
DOI:
https://doi.org/10.33474/jki.v10i1.11191Keywords:
Jati Belanda, Kemuning, Murbei, Rimpang Bangle, Hepatic SteatosisAbstract
Fatty liver is an accumulation of lipid drops exceeding 5% of the total amount of liver, which can increase the risk of nonalcoholic fatty liver disease (NAFLD). NAFLD is associated with dyslipidemia, which the treatments for both condition are in common. Jati Belanda leaves, Kemuning leaves, Murbei leaves and Bangle rhizome (JKMB) are often used alone or in combination to improve dyslipidemia, but their effect on the liver is unknown.
This study used an experimental post test control group method where 25 rats were acclimatized for 2 weeks. After that, the rats were divided into 5 groups including KN : normal feed, KP : DTLM feed, D1 : DTLM+ JKMB extract dose 189 mg/200gbb, D2 : DTLM+ JKMB extract dose 378 mg/200gbb, D3 : DTLM+ JKMB extract dose 756 mg /200gbb and treated for 12 weeks. JKMB extract was obtained in a ratio of 8: 3: 3: 7. After that, the rats were sacrificed and the liver tissue was stained with Hematoxylin-Eosin staining. HE preparations were performed to calculate the percentage of liver fat at 400x magnification with 5 fields of view per slide.
The results of the normality (0.200> 0.05) and homogeneity test (0.558> 0.05) were found to be normal. The ANOVA test was found to be significantly different (0.000< 0.05). The Post Hoc test found that the negative control was significantly different from the positive control (0.000 < 0.05). Positive control did not differ significantly with treatment doses D1 (0.094>0.05), D2 (0.186>0.05) and D3 (0.734>0.05).
From the results of the research above, it can be concluded that the combination of extracts of Jati Belanda leaves, Kemuning leaves, Murbei leaves and Bangle rhizome extracts could not reduce the percentage of fatty liver.References
Antonio, A., Perea, V. 2019. Dyslipidemia in nonalcoholic fatty liver disease. Current Opinion in Endocrinology & Diabetes and Obesity. Vol. 26 (2):103-108
Arumsari, B.R. 2014. Efek Ekstrak Etanol Daun Kemuning (Murraya paniculata (L.) Jack) terhadap Kadar Kolesterol HDL Tikus Wistar Jantan. Undergraduate thesis, Universitas Kristen Maranatha. http://repository.maranatha.edu/9042/
Batubara, I., Husnawati, H., Darusman, L.K., Mitsunaga, T. 2017. Senyawa Penciri Eks- trak Daun Jati Belanda (Guazuma ulmi- folia Lamk) sebagai Anti-Kolesterol. Jurnal Ilmu Pertanian Indonesia Vol. 22(2): 87-91.https://journal.ipb.ac.id/index.php/JIPI/a rticle/view/17519/12686
Bellentani, S., Scaglioni, F., Marino, M., Bedogni,G. 2010. Epidemiology of Non-Alcoholic Fatty Liver Disease. Digestive Diseases 28(1):155-61
Chatrath, H., Vuppalanchi, R., Chalasani, N. 2012. Dyslipidemia in Patients with Non- alcoholic Fatty Liver Disease. Semin Liver Dis. 32(1): 22–29. https://www.ncbi.nlm.nih.gov/pmc/articl es/PMC3654545/
Gitawati, R., Widowati, L., Suharyanto, F. 2015. Penggunaan Jamu pada Pasien Hiperlipi- demia Berdasarkan Data Rekam Medik, di Beberapa Fasilitas Pelayanan Kesehatan di Indonesia. Jurnal Kefarmasian Indone- sia Vol.5(1):41-48. https://ejournal2.litbang.kemkes.go.id/in dex.php/jki/article/view/3474/1649
Hidajat, M., Aman, I. G. M., Pangkahila, A., Sukoco, H., Siswanto, F. M. 2019. Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia. Jurnal Sains dan Teknologi Peternakan Vol.1(1):25-30. https://ojs.unsulbar.ac.id/index.php/jstp/article/view/541/292
Hosseinikia, M., Oubari, F., Hosseinkia, R., Tabeshfar, Z., Salehi, M.G., Mousavian, Z., Abbasi, M., Samadi, M. and Pasdar, Y. 2020. Quercetin supplementation in non- alcoholic fatty liver disease: A randomi- zed, double-blind, placebo-controlled clinical trial. Nutrition & Food Science. Vol. 50(6): 1279-1293. https://doi.org/10.1108/NFS-10-2019- 0321
Huang, J., Wang, Y., Ying, C., Liu, L., Lou, Z. 2018. Effects of mulberry leaf on experimental yperlipidemia rats induced by high‑fat diet. Experimental And Therapeutic Medicine 16: 547-556. https://www.spandidos- publications.com/10.3892/etm.2018.625 4
Jayanti, V.D. 2007. Pengaruh Pemberian Infusa Daun Jati Belanda (Guazuma ulmifolia Lamk.) terhadap Kadar Trigliserida dalam Plasma Tikus Putih Jantan Galur Wistar. Skripsi. Universitas Sanata Dharma. https://repository.usd.ac.id/16768/2/038 114105_Full.pdf
Jeong, S. M., Kang, M. J., Choi, H. N., Kim, J. H., Kim, J. I. 2012. Quercetin ameliorates hyperglycemia and dyslipidemia and improves antioxidant status in type 2 diabetic db/db mice. Nutr Res Pract. 6(3): 201–207. https://www.ncbi.nlm.nih.gov/pmc/articl es/PMC3395784/
Kardela, W., Fauziah, F., Nadia, S. 2019. Uji Aktivitas Fraksi Ekstrak Etanol Daun Kemuning (Murraya paniculata (L.) Jack) Tehadap Kadar Glukosa Darah dan Indeks Aterogenik Tikus Putih Jantan. Jurnal Farmasi Higea, Vol. 11, (1) 83-90. http://www.jurnalfarmasihigea.org/inde x.php/higea/article/view/218
Lee, M. R., Ki, J. E., Park, J.W ., Kang, M. J., Choi,H. J., Bae, S. J., Choi, Y. W., Kim , K. M., Hong, J. T., Hwang, D. Y. 2020. Fermented mulberry (Morus alba) leaves suppress high fat diet-induced hepatic steatosis through amelioration of the inflammatory response and autophagy pathway. BMC Complement Med Ther. 18; 20 (1): 283. https://pubmed.ncbi.nlm.nih.gov/32948 162/
Mallaleng, H. R., Purwaningtyas, U., Hermawati, R., Solicha, N., Zulkarnain, F. 2015. Tanaman Obat untuk Penyakit Sindrom Metabolisme. Malang. Universitas Negeri Malang.
Nassir, F., Rector, R. S., Hammoud, G. M., Ibdah, J. A. 2015. Pathogenesis and Prevention of Hepatic Steatosis. March Gastroentero- logy and Hepatology 11(3):167-175. https://www.researchgate.net/publicatio n/281759175_Pathogenesis_and_Preven tion_of_Hepatic_Steatosis
Nugroho, D. D. 2014. Pengaruh Ekstrak Daun Jati Belanda (Guazuma ulmifolia lamk) Terhadap Penurunan Kadar Kolesterol LDL Manusia. Skripsi. Universitas Kristen Maranatha. http://repository.maranatha.edu/12764/ 10/1110164_Journal.pdf
Petta, S., Gastaldelli, A., Rebelos, E., Bugianesi, E., Messa, P., Miele, L., Svegliati-Baroni, G., Valenti, L, Bonino, F. 2016. Pathophysiology of Non Alcoholic Fatty Liver Disease. Int J Mol Sci. 17(12): 2082.
Rahayaan, N. R. H. 2018. Efektifitas Infus Daun Jati Belanda (Guazuma Ulmifolia Lamk.) Terhadap Penurunan Kadar Kolesterol To- tal Tikus Wistar Jantan yang Diberi Pakan Tinggi Lemak. Undergraduate thesis, Universitas Kristen Maranatha.
Rahmawati, N., Mustofa, F.I., Haryanti, S. Mujahid, R. 2021. Medicinal plant utilization for hypercholesterolemia by traditional healers in Java island. IOP Conf. Series: Earth and Environmental Science 637. https://iopscience.iop.org/article/10.108 8/1755-1315/637/1/012043/pdf
Sufyan, D. L., Dwi, A., Prijadi, B. 2019. Pengaruh Pemberian Jus Terong Ungu terhadap Perlemakan Hati Tikus Wistar. Jurnal Ilmiah Kesehatan, 18 (2) : 59-63.
Sukandar, E. Y., Elfahmi, Nurdewi. 2009. Pengaruh Pemberian Ekstrak Air Daun Jati Belanda (Guazuma Ulmifolia Lamk.) terhadap Kadar Lipid Darah pada Tikus Jantan. JKM. Vol.8 (2): 102-112.
https://docplayer.info/34661093- Pengaruh-pemberian-ekstrak-air-daun- jati-belanda-guazuma-ulmifolia-lamk- terhadap-kadar-lipid-darah-pada-tikus- jantan.html
Sukohar, A., Setiawan, G., Morfi, C. W., Graharti, R., Wahyudo, R., Sabilla, C. T. 2018. The Effect of Kemuning Leaves Infusion (Murraya Paniculata (L.) Jack) on the Lipid Profile of Obese Patients. Biomedical & Pharmacology Journal Vol. 11(1), 417-422
Ulfah, V. F., Iskandar, Y. 2019. Review Jurnal: Aktivitas Tanaman Jati Belanda (Guazuma ulmifolia Lam.) sebagai Antihiperlipide-mia. Farmaka Suplemen Vol. 17 (1). http://jurnal.unpad.ac.id/farmaka/article/view/22219/pdf
Wardani E, Harahap Y, Mun’im A, Bahtiar A. 2019. Influence of Extraction on the Yield, Phytochemical, and LCMS Profile from Standardized Kemuning Leaf (Murraya paniculata (L.) Jack). Pharmacog J. 2019; 11 (6) Suppl: 1455-1462. https://www.researchgate.net/publicatio n/338116674_Influence_of_Extraction_o n_the_Yield_Phytochemical_and_LCMS_ Profile_from_Standardized_Kemuning_L eaf_Murraya_paniculata_L_Jack (16) (PDF) Influence of Extraction on the Yield, Phytochemical, and LCMS Profile from Standardized Kemuning Leaf (Murraya paniculata (L.) Jack). Available from: https://www.researchgate.net/publicatio n/338116674_Influence_of_Extraction_o n_the_Yield_Phytochemical_and_LCMS_ Profile_from_Standardized_Kemuning_L eaf_Murraya_paniculata_L_Jack [accessed Jun 07 2021].
Wening Sari, W. 2019. Ethanolic Extracts of Mulberry (Morus alba Linn) Leaf Prevent Hyperlipidemia and Oxidative Stress- induced Steatohepatitis in Rats. International Summit on Science Technology and Humanity (ISETH2019). https://publikasiilmiah.ums.ac.id/bitstrea m/handle/11617/11823/61.pdf?sequenc e=1&isAllowed=y
Zeni, A. L. B., Dall'Molin, M. 2010. Hypotriglyceridemic effect of Morus alba L., Moraceae, leaves in hyperlipidemic rats. Rev. bras. farmacogn. 20 (1):130-133. https://www.scielo.br/j/rbfar/a/Nxdc6kC LFN4p5VVyVQPJXQb/?format=pdf&lang= en
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