EFEK PENGGUNAAN POLYETHYLEN GLYCOL (PEG) DALAM RANSUM KONSENTRAT MENGANDUNG DAUN KABESAK PUTIH (Acacia leucophloea) TERHADAP PRODUKSI GAS DAN TOTAL VFA IN VITRO
DOI:
https://doi.org/10.33474/rekasatwa.v6i1.21709Keywords:
daun kabesak putih, konsentrat, polyethylene glycol, produksi gas, in vitroAbstract
Tujuan dari penelitian ini adalah menentukan efek polyethylene glycol (PEG) sebagai penghambat aktivitas tannin dalam ransum konsentrat mengandung daun kabesak putih terhadap produksi gas in vitro dan produksi total asam lemak terbang (T-VFA). Penelitian menggunakan metoda teknik produksi gas. Perlakuan yang diterapkan adalah level penggunaan daun kabesak putih dalam ransum konsentrat dengan penambahan PEG. Perlakuan dimaksud adalah : R1: 10% daun kabesak putih, R2 : 20% daun kabesak putih, R3 : 30% daun kabesak putih, dan R4: 40% daun kabesak putih. Hasil penelitian menunjukkan perlakuan berpengaruh nyata terhadap produksi gas dan total VFA ransum. Potensi produksi gas (mL/500 mg BK) dan laju produksi gas fraksi tak larut (mL/jam), dan produksi gas 48 jam (mL/500 mg BK) tidak menunjukkan perbedaan diantara perlakuan. Produksi T-VFA menunjukkan perlakuan R1 dan R2 nyata lebih tinggi dibanding perlakuan lainnya. Disimpulkan bahwa Penambahan Polyethylene glycol (PEG) dalam ransum konsentrat mengandung daun kabesak putih meningkatkan produksi gas in vitro dan produksi asam lemak terbang (T-VFA). Keberadaan tannin dalam daun kabesak putih dapat dihambat dengan keberadaan PEG sehingga fermentasi ransum menjadi meningkat.
References
Alonzo-Diaz, M.A., J.F.J. Torres-Acosta., C.A. Sandoval-Castro and H. Hoste. 2010. Tannins in tropical tree fodders fed to small ruminants: A friendly foe? Small Rum. Resc. 89:164-173.
A.O.A.C. 2000. Official Methods of Analysis of the Association of Official Analytical Chemist.11th ed. Washington D.C.
Bagheripour, E., Y. Rouzbehan and D. Alipour. 2008. Effects of ensiling, air-drying, and addition of polyethylene glycol in vitro gas production of pistachio by-products. Anim. Feed Sci. Technol., 146:327-336. http://dx.doi.org/10.1016/j.anifeedsci.2008.01.002.
Besharati, M. and A. Taghizadeh. 2011. Effect of tannin-binding agents (polyethylene glycol and polyvinylpyrrolidone) supplementation on in vitro gas production kinetics of some grape yield byproducts. International Scholarly Research Network (ISRN). 1-8. Doi:10.5402/2011/780540.
Calabro S, A. Guglielmelli, F. Lannaccone, P. P. Danieli, R. Tudisco, C. Ruggiero, G. Piccolo, M. I. Cutrignelli and F. Infascelli. 2012. Fermentation kinetics of sainfoin hay with and without PEG. Journal of Animal Physiology and Animal Nutrition 96:842–849.
Davies, Z.S., D. Mason, A.E. Brooks, G.W. Griffith, R. J. Merry and M.K. Theodorou. 2000. An automated system for measuring gas production from forage incubated with rumen fluid and its use in determining the effect of enzymes on grass silage. Anim. Feed Sci. Technol., 83: 205-221.
Djogo, T. 1988. Penganekaragaman Tanaman Pengganti Lamtoro dengan Tanaman Lokal Tahan Kering untuk Perhutanian (Agroforestry) di Kabupaten Kupang. Dalam: Prinsip-prinsip Produksi dan Metode Penelitian Peternakan. Kumpulan Materi Kursus. Sub Balai Penelitian Ternak, Lili-Kupang bekerjasama dengan Projek P3NT : 139-154.
Dung, N.T., N.T. Mui and I. Ledin. 2005. Effect of replacing a commercial concentrate with cassava hay (Manihot esculenta Crantz) on the performance of growing goats. Anim. Feed Sci. Technol., 119:271-281.
Getachew, G., H.P.S. Makkar and K. Becker. 2000. Effect of polyethylene glycol on in vitro degradability of nitrogen and microbial protein synthesis from tannin-rich browse and herbaceous legumes. Brit. J. Nutr., 84:73-83.
Getachewa, G, G.M. Crovetto, M. Fondevila, U. Krishnamoorthy, B. Singh, M. Spanghero, H. Steingass, P.H. Robinson, M.M. Kailas. 2002. Laboratory variation of 24 h in vitro gas production and estimated metabolizable energy value of ruminant feeds. Animal Feed Sci Technol. 102:169-80. https://doi.org/10.1016/S0377-8401(02)00212-2.
Makkar, H.P.S. and K. Becker. 1993. Vanillin-Hcl method for condensed tannins: Effect of organic solvents used for extraction of tannins. Chem Ecol;19(4): 613-21.
Makkar, H.P.S., M. Blümmel and K. Becker. 1995. Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implications in gas production and true digestibility in In vitro techniques. Br. J. Nutr., 73: 897-933.
Makkar, H.P.S. 2005. Use of nuclear and related techniques to develop simple tannin assays for predicting and improving the safety and efficiency of feeding ruminants on tanniniferous tree foliage. Summary of the FAO/IAEA Coordinated Research Project. Anim. Feed Sci. Tech. 122:3-12.
Mlambo, V., J.L.N. Sikosana., F.L. Mould., T. Smith., E. Owen and I. Mueller-Harvey. 2007. The effectiveness of adapted rumen fluid versus PEG to ferment tannin-containing substrates in vitro. Anim. Feed Sci. Tech., 136:128-136.
Mueler-Harvey, I. 2006. Unraveling the Conundrum of tannins in animal nutrition and health. J. Sci. Food. Agric., 86 : 2010-2037.
Olivares-Peres, J., F. Aviles-Nova., S. Rojas-Hernandez., B. Albarran-Portilo and O.A. Castelan-Ortega. 2011. Identification uses and measurement of offers legumes trees in south farmers of the State of Mexico. Tropical and Subtropical Agroecosystems., 14:739-748. http://www.redalyc.org/articulo.oa?id=93931761006.
Olivares, P.J., N.F. Aviles., P.B. Albarran., O.O.A. Castelan and H.S. Rojas. 2013. Use of three fodder trees in the feeding of goats in the subhumid tropics in Mexico. Tropical Animal Health and Production., 45:821-828.
Orskov, E.R. and I. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. Cambridge., 92:499-503.
Orwa, C.A., A. Mutua., R. Kindt., Jamnadass and A. Simons. 2009. Agroforestry database: a tree reference and selection guide version 4.0.
Rodriquez, R., N. Gonzalez, A. Ramirez, S. Gomes, O. Moreira, L. Sarduy and Y. Medina. 2014. Tannins of tropical shrub-like legumes: their effect on protein protection of soybean meal. Cuban J. Agric. Sci. 48(3): 247-252.
SAS. 1998. User’s Guide: Statistics, Version 7th Edition. SAS Inst. Inc., Cary, NC.
Sallam, S.M.A.H., I.C. da Silva Bueno., P.B. de Godoy., E.F. Nozella., D.M.S.S. Vitti and A.L. Abdalla. 2010. Ruminal fermentation and tannins bioactivity of some browse using a semi-automated gas production technique. Tropical and Sub Tropical Agroecosystems.,12:1-10. http://www.redalyc.org/articulo.oa?id=93913074001.
Seresinhe, T. & C. Iben. 2003. In vitro quality assessment of two tropical shrub legumes about their extractable tannins content. J. Anim. Physiol. Anim. Nutr. 87, 109-115.
Traiyakun, S., W. Harakord., C. Yuangklang and P. Paengkoum. 2011. Leucaenleucocephala meal as a replacement for soybean meal in growing goat diets. J. Agric. Sci. and Technol. A., 1:1150-1154.
Van Soest, P. J., and J. B. Robertson 1976. Chemical and physical properties of dietary fibre. Page 13 in Proc. Miles Symp. Nub. Soc. Can. Halifax, NS, Can.
Waghorn, G.C., A. John., W.T. Jones, and I.D. Shelton. 1987. Nutritive value of Lotus corniculatus L. containing low and medium concentrations of condensed tannins for sheep. Proc. N.Z. Soc. Anim. Prod., 47 : 25-30.
Waghorn, G. 2008. Beneficial and detrimental effects of dietary condensed tannins for sustainable sheep and goat production-progress and challenges. Anim. Feed Sci. and Tech., 147:116-139. www.elsevier.com/locate/anifeedsci.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Dr, Ir. Emma Dyelim Wie Lawa, MP
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
_-_Copy1.jpg)
